Santiago Castro, Federico Bernardeschi and Jonathan Rowe lead the attack, as Bologna hope to build on a 1-0 Europa League play-off lead with Brann.
It kicks off at the Stadio Dall’Ara at 20.00 GMT (21.00 CET).
The Rossoblu had been in a deep crisis, but managed to turn their campaign around with three consecutive victories in all competition, including a 1-0 first leg result in Norway.
That is a strong start, but not enough to feel secure, especially as their home record has been so poor lately.
There is not a lot of squad rotation, so Castro is flanked by Bernardeschi and Rowe, with Lewis Ferguson in midfield.
Juan Miranda and Charalampos Lykogiannis are injured, while Benjamin Dominguez is in the squad after shaking off flu symptoms.
Brann already visited the Stadio Dall’Ara during the league phase this season, emerging with a 0-0 draw in November, despite playing against 10 men for over an hour.
Niklas Castro and Saevar Atli Magnusson are long-term absentees.
The winners will face either Roma or Freiburg in the Round of 16, with the draw held on February 27.
Bologna vs. Brann line-ups
Bologna: Skorupski; Zortea, Lucumi, Vitik, Joao Mario; Moro, Freuler, Ferguson; Bernardeschi, Castro, Rowe
Brann: Dyngeland; De Roeve, Pallesen, Boakye, Soltvedt; Ingason, Sorensen, Myhre; Mathisen, Holm, Thorsteinsson
Ref: Bastien (FRA)
Player statistic
| Martin Vitik |  |
28' | ||
| 37' |  |
Jacob Lungi Sörensen | ||
| Federico Bernardeschi | |
41' |
| João Mário |  |
56' | ||
| 78' | |
Rasmus Holten | ||
| Riccardo Orsolini | |
80' |
Match statistic
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